Extended Data Fig. 6: Phenotypic characterization of OT-II pTregs in peripheral lymphoid organs and the gut.
From: Facile induction of immune tolerance by an interleukin-2–TGFβ surrogate agonist
a–d, OT-II cell phenotypes after gut migration (n = 10 mice/group). a, Schematic illustration of OVA administration in the drinking water. Created in BioRender; Sun, Q. https://BioRender.com/0njaeg9 (2026). b–d, Representative flow cytometry plots (b) and quantification of the frequencies (c) and numbers (d) of the indicated OT-II cell subsets in the indicated tissues. e–i, Phenotypic analysis of total and pTreg OT-II cells (n = 16 samples/group). e, Gating strategy for identifying distinct subsets among donor OT-II cells. f, Representative flow cytometry plots showing IFN-γ and IL-17a production by donor OT-II cells in LNs. g, Quantification of TNF-α+ OT-II cell frequencies in LNs. h, i, Representative flow cytometry plots and quantification showing expression of the indicated molecules in the indicated CD4+ T cell subsets from LNs. Data are presented as mean ± s.e.m. The data in a–i are representative of two independent experiments. The statistics were obtained by unpaired Welch’s t-test (two-tailed) (c and d) or one-way ANOVA coupled with Tukey’s multiple-comparisons test (g).
