Extended Data Fig. 10: Contribution of R1855 to pre-miRNA dicing outcomes.
From: DICER cleavage fidelity is governed by 5′-end binding pockets
a, The presence of mWCU and YCR motifs in pre-mir-629 and pre-mir-517a. b, The in vitro dicing assays. Left, denaturing gels of in vitro dicing with WT DICER and R1855 mutants (R1855A/E/K) using shRNA substrates that variably include the YCR motif, mWCU motif, and/or extended stem. Right, schematics of substrates and quantification of cleavage accuracy (DC21, DC22, others) from the three independent repeats.
