Fig. 3: ZBTB18 depletion reduces callosal and subcerebral projections while increasing cortico-cortical association projections.
From: Adaptive evolution of gene regulatory networks in mammalian neocortex
a, Visualization of axonal projections by GFP expression in the Neurod6–Cre; Zbtb18 cKO mouse brain. Full arrows and arrowheads depict wild type, and open arrowheads depict defective tracts in the Zbtb18 cKO brain. b, Whole brain image (left top) and coronal sections of the brain at P7 showing the injection site and extent of the GFP expression after AAvrg–CAG–Gfp injections into the medial PFC at PD 3. c, Left, bar plot showing GFP-positive neurons projecting to PFC in the neocortex of Zbtb18 cKO mice compared with control brains at PD 7, as shown in b. Unpaired two-tailed t-test. The graph represents mean ± s.e.m. *P = 0.03 (n = 3). Right, line graph data showing the percentage distribution of GFP+ neurons projecting to PFC in each bin of the neocortex of Zbtb18 cKO mice compared with wild-type brains at PD 7. The number of labelled neurons in each bin was compared using unpaired t-test. The graph represents mean ± s.e.m. *P = 0.03 (bin 3), 0.004031 (bin 6), 0.03 (bin 7) and 0.012 (bin 14) (n = 3). AC, anterior commissure; AUD, auditory cortex; BLA, basolateral amygdala (excitatory nucleus within amygdala); CC, corpus callosum; CP, cerebral peduncle; cPERI, contralateral perirhinal; CST, cortico-spinal tract; Hip, hippocampus; IC, internal capsule; nLOT, nucleus of lateral olfactory tract; PERI, perirhinal cortex; Str, striatum; Th, thalamus. Scale bars, 1 mm (a), 20 µm (a (CP panel)), 500 μm (b), 125 μm (b (inset)).
