Extended Data Fig. 4: ZBTB18 Expression in the Developing Cerebral Cortex of Humans and Mice.
From: Adaptive evolution of gene regulatory networks in mammalian neocortex
a) Coronal sections showing the immunolabeling for three anti- ZBTB18 antibodies on Neurod6-Cre; Zbtb18 cKO as compared to the control. Arrow points to the expression of ZBTB18 in the ventral region of the cKO where Neurod6-Cre appears not be active. Scale bar: 150 µm. b) Zbtb18 mRNA are co-expressed with Cux2 mRNA in PD 0 mouse neocortex in L2-3 (arrows) as well as in L5 (arrowheads). Scale bar: 1 mm, 20 µm (right). c) ZBTB18 mRNA is co-expressed with CUX2 mRNA (arrow) in 20 PCW human frontal cortex. Scale bar: 2 mm, 40 µm (right) CP, cortical-plate; IZ, intermediate zone; SVZ, subventricular zone; VZ, ventricular zone. All images of mouse neocortex are representative of at least 3 independent biological replicates. In situ hybridization of a postmortem postconception weeks (PCW 20) human neocortex reflects multiple sections from a single brain but is consistent with an additional PCW 18 postmortem human brain sample (not shown).
