Extended Data Fig. 9: Features of adenomas following colitis.
a, Distribution of macroscopic adenoma size between control and colitis recovered animals. Each point represents a single tumor. P-value from K-S test. b, Proliferation in adenomas following high dose tamoxifen induction (50 mg/kg x 3). n = 3 mice per condition. c, Clonogenicity in adenoma-derived organoid lines. n = 3 control and 4 colitis recovered organoid lines. d, Visible adenoma number following high dose tamoxifen induction. n = 4 control and 5 colitis recovered mice. e, Representative example of microscopic tumor following low dose tamoxifen induction (10 mg/kg x 1). Scale bar 100 μm. f, Method for computing tumor area. Left, low magnification image used to compute total tissue area. Middle, high magnification image where individual tumors are individually traced. Right, equation for computing percent tumor area. g, Quantification of microscopic tumor area following low dose initiation. Each point represents the total area per animal as a percentage of total tissue assessed. n = 7 control mice and 10 colitis recovered mice. h, Microscopic tumor number normalized to total tissue area. n = 7 control mice and 10 colitis recovered mice. i, UMAP embedding of scATAC-seq data of primary tissue following adenoma induction with cells colored by cluster. j, UMAP embeddings showing Lef1 motif accessibility (top), Axin2 relative gene expression (middle), and sample condition (bottom). k, Marker gene expression for each epithelial cell type identified. For each gene, the size of the circle indicates the fraction of cells in which at least one transcript was detected and the color indicates average expression within the cell type. l, Beta-catenin immunohistochemistry images of microscopic tumors following in vivo AP-1 inhibition. Circles indicate examples of lesions. Scale bar 100 μm. Representative of n = 6 control vehicle-treated, 7 colitis recovered vehicle-treated and 7 colitis recovered T-5224-treated mice. All error bars are s.e.m.
