Extended Data Fig. 2: Independent studies on gene expression, chromatin accessibility, tissue survival, and immunofluorescence.
From: Dominant clones leverage developmental epigenomic states to drive ependymoma
(a) Confirmation of Plag1, Plagl1, and Plagl2 overexpression in mRGCs by RNA-seq. (b) Heatmap of ZR 93 gene signature for parental, RFP, Plag1, Plagl1, Plagl2, and ZR expressing mRGCs by RNA-seq. (c) Survival curve for GLAST-PBase, pBCAG-ZR (red, n = 17) vs GLAST-PBase, pBCAG-ZR, Plagl1 KO IUE mice (blue, p = 0.8211). (d) Survival curve for mRGC ZR+ (blue, n = 5) vs mRGC ZR+ Plagl1 KO (red, n = 5) implanted mice (p = 0.7778). (e) Confirmation of Plagl1 KO for mice within survival curves. Red X denotes a mouse with no knockout which was removed from analysis. (f) Chromatin accessibility signals for Fabp7, Plagl1, and Notch1 are detected by spatial scATAC in cells located around the ventricles of mouse embryos. Figure adapted from Zhang et al. Nature 2023. (g) Immunofluorescence for GFP in P2 mice after GFP IUE. Arrows denote radial fibers typical of RGCs.
