Extended Data Fig. 5: snrk1β1a mutants show enhanced resistance in rice fields.
From: Inactivating SnRK1β1A promotes broad-spectrum disease resistance in rice
a, Identification of transgene-free snrk1β1a mutants. Primers specific to the Cas9, HPT and Actin genes, respectively, were used in genotyping. The Actin gene was used as the DNA quality control. b, snrk1β1a lines showed significantly reduced leaf blast disease indices than their WT ZH11 in rice fields of Shangzhuang (SZ), Panjin (PJ), Donggang (DG) and Enshi (ES). c–e, snrk1β1a lines were resistant to panicle blast, sheath blight and false smut disease in the DG nursery. The white triangle in (d) indicates the symptoms of sheath blight disease and the black triangle in (e) indicates the symptoms of false smut ball. (b–e) were from natural infection with multiple duplicates. Data represent mean ± s.d.; n = biologically independent samples in the graphs. Two-tailed Student’s t tests were employed in (b–e), **P < 0.01. For exact P values, see Source Data. Experiments were repeated three times in (b–e) with similar results.
