Extended Data Fig. 1: Generation and characterization of the Oprm1Cre knock-in mouse line.
From: Deconstruction of a spino-brain–spinal cord circuit that drives chronic pain
a, Gene targeting strategy used to generate the Oprm1Cre knock-in mouse line. b, Morphine produced similar analgesic effect in WT (black) and Oprm1Cre/+ (blue), but not in Oprm1Cre/Cre (red) mice (Non-parametric ANOVA (Friedman test), post-hoc pairwise comparisons were conducted using Dunn’s multiple comparison test. WT Saline vs. 2.5 mg/kg morphine: P = 0.1733; WT Saline vs. 5 mg/kg morphine: P = 0.0133; WT 2.5 mg/kg morphine vs. 5 mg/kg morphine: P > 0.9999; Oprm1Cre/+ Saline vs. 2.5 mg/kg morphine: P = 0.6177; Oprm1Cre/+ Saline vs. 5 mg/kg morphine: P = 0.0080; Oprm1Cre/+ 2.5 mg/kg morphine vs. 5 mg/kg morphine: P = 0.2460; Oprm1Cre/Cre Saline vs. 2.5 mg/kg morphine: P > 0.9999; Oprm1Cre/Cre Saline vs. 5 mg/kg morphine: P > 0.9999; Oprm1Cre/Cre 2.5 mg/kg morphine vs. 5 mg/kg morphine: P > 0.9999. n = 5 mice for each genotype). c, Morphine increased locomotion in WT (black) and Oprm1Cre/+(blue), but not in Oprm1Cre/Cre (red) mice. Two-sided Wilcoxon signed-rank test (WT saline vs. WT 15 mg Kg−1 morphine, P = 0.0313; Oprm1Cre/+ saline vs. Oprm1Cre/+ 15 mg Kg−1 morphine, P = 0.0313; Oprm1Cre/Cre saline vs. Oprm1Cre/Cre 15 mg Kg−1 morphine, P = 0.0625). n = 6 mice for WT and Oprm1Cre/+, n = 5 mice for Oprm1Cre/Cre. d, Representative images from three independently performed experiments show the Ruby3-expressing terminal in the thalamus from OPRM1+ ascending RVM neurons after injection of AAV9-FLEX(LoxP)-Ruby3 into the RVM of the Oprm1Cre mice. Scale bar: 1 mm. * P < 0.05, ** P < 0.01. Data are presented as mean ± s.e.m.
