Extended Data Fig. 1: Map-based cloning identifies Xa48.
From: Asymmetric selection of a rice immune module and rebuild of disease resistance
a, SKZ displayed strong resistance to J18 but high susceptible to PXO99A. Disease symptoms of SKZ, NIPB, TP309, and 106 at 14 dpi with Xoo strains J18 and PXO99A (n = 36). Line 106 is transgenic TP309 expressing Xa21. b, Xa48 was initially mapped to chromosome 3 between two InDel markers InDel26 and InDel47. c, d, Independent CRISPR/Cas9 knockout lines (CR-C1, CR-C2, CR-C3, CR-C4) of SKZ were generated (c), and representative lines’ lesion length of knockout mutants were measured (d). Note that only CR-C1 became susceptible to J18. e, Premature termination of XA48 translation in susceptible varieties caused by a single-base deletion and SNP. XA48 encodes a typical CNL receptor consisting of 1,094 amino acids, with N-terminal coiled-coil (CC) domain (1-195), NB-ARC domain (215-440), and C-terminal leucine-rich repeat (LRR) domain (555-1012). f, Transcriptional level of XA48 was detected in TP309-Xa48 and NIPB-Xa48 complement plants (two-tailed t test, n = 3). g, h, NIPB-Xa48 transgenic plants are highly resistant to J18 as SKZ. The lesion length (g) and bacterial populations (h) were measured. Data are shown as boxplots displaying the distribution of individual values (dots). Error bars represent the estimated marginal means (EMM) ± SE from linear mixed-effects models (a, d, g) or arithmetic means ± SD (f, h). For a, d, g, EMMs derived from linear mixed-effects models fitted to data from two or three independent experiments are compared between each genotype and the wild-type control using two-tailed planned contrasts. Scale bars, 5 cm.
