Extended Data Fig. 10: Diversity of cell types within a motor cortex astrocyte network.
From: Astrocytes connect specific brain regions through plastic networks
a, b, c. Individual slices at varied depths in the horizontal plane through a light sheet imaged brain infected in motor cortex (yellow lines in reference 3D image, top right; HA, cyan; Streptavidin, magenta). a’. Biotinylated compounds fill many, but not all, adjacent cells. a”. While many brain regions show signal bilaterally, some regions, such as prefrontal cortex in this brain, do not. b’. Following motor cortex infection, sparse fluorescence in some neurons is visible. b”. Chains of astrocytes contact and diverge from neuronal axons, which qualitatively become brighter following astrocyte network contact points. b”’. Most astrocyte network termini, such as this terminus in prefrontal cortex, do not show detectable streptavidin signal among adjacent neurons. c’. Following motor cortex infection, axons descending to the spinal cord show visible streptavidin signal.
