Extended Data Fig. 9: Size reduction of 76E1 to scFv reverses antibody evasion caused by H655Y mutation-mediated epitope shielding and viral entry pathway change.
From: Steric hindrance of antibody binding in an Omicron spike fusion intermediate
a, Neutralizing curves of 76E1-FL, Fab and scFv at equal molar concentrations against pseudoviruses of SARS-CoV-2 WT, WT-H655Y and reverted Y655H in BF.7 in 293T-ACE2 cells. b-c, Neutralization curves of 76E1-FL (b) and scFv (c) against SARS-CoV-2 WT, WT-H655Y, BF.7 and BF.7-Y655H pseudoviruses in 293T-ACE2 and Caco-2 cells, respectively. a-c, n = 3. Data: mean ± s.e.m; representative of two experiments.
