Extended Data Fig. 2: Sample purification and cryo-EM workflows for identifying distributions of various species from different datasets. | Nature

Extended Data Fig. 2: Sample purification and cryo-EM workflows for identifying distributions of various species from different datasets.

From: Steric hindrance of antibody binding in an Omicron spike fusion intermediate

Extended Data Fig. 2: Sample purification and cryo-EM workflows for identifying distributions of various species from different datasets.The alternative text for this image may have been generated using AI.

a, Size exclusion chromatography, SDS-PAGE, and negative stain EM micrographs of wild-type (WT, black) and XBB.1.5 (orange) S. Data are representative of 3 experiments. b-c, Size exclusion chromatography and SDS-PAGE of soluble ACE2 (b) and 76E1-Fab (c). Data are representative of 2 experiments. d-f, Data processing workflows for identifying distributions of various species from the randomly generated sub-datasets from dataset 1 (WT-ACE2-76E1, 30 min) (d), dataset 2 (XBB.1.5-ACE2-76E1, 10 min) (f), and dataset 3 (XBB.1.5-ACE2-76E1, 30 min) (e).

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