Extended Data Fig. 4: Cryo-EM workflow and map quality analysis of XBB.1.5 S-ACE2-76E1 Fab complex.
From: Steric hindrance of antibody binding in an Omicron spike fusion intermediate
a, Cryo-EM data processing workflow. The final cryo-EM maps are colored according to the local resolution estimated by cryoSPARC with the FSC = 0.143 criterion. b, Gold standard FSC curves of the refined 3D reconstructions in XBB.1.5 SE-FIC-76E1. c, The XBB.1.5 SE-FIC-76E1 structure fits into the 5.65 Å map. d, The S2′-helix in IL770 and variable regions of 76E1-Fab fit into the local S2-76E1 map at a resolution of 4.14 Å. e-h, Representative density in gray surface representation from the local S2-76E1 map. Colors of various components in (c-h) correspond to those in Extended Data Fig. 3d–i, respectively.
