Extended Data Fig. 2: The enrichment of splicing machinery at Flower exon 3 is specific to HGSC stromal tissue. | Nature Biotechnology

Extended Data Fig. 2: The enrichment of splicing machinery at Flower exon 3 is specific to HGSC stromal tissue.

From: Ovarian tumor cells gain competitive advantage by actively reducing the cellular fitness of microenvironment cells

Extended Data Fig. 2: The enrichment of splicing machinery at Flower exon 3 is specific to HGSC stromal tissue.

A) A heatmap depicting relative enrichment from ChIP influenced gene walking experiment showing association of DNA methylation, G9a, GLP, H3K9Me3, HP1, SRSF3, and RNA Pol II, on each exon (1-6) and 200bp flanking regions upstream (5’) and downstream (3’) from each Exon of the hFwe gene locus. The bar representing the value ranges 0.3 and 4 (color yellow-green-blue) illustrates the relative binding/association of the epigenetic markers (left column) on the respective exons 1-6 and 200bp 5’ & 3’ flanking regions (Biological replicates, n=8). B) Methylight assay performed in all hFwe exon bodies in normal, HGSC tumor, and stromal samples showing specific and exclusive methylation of hFwe exon 3 in HGSC stromal tissue. Data are presented as mean values +/- SEM, (Biological replicates, n=15).

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