Extended Data Fig. 6: RNA-PLA analysis of RNA-protein pairs involving LINC00339 noncoding RNA.
From: Genome-wide mapping of RNA-protein associations through sequencing
(a–c) Representative images of the tests (protein name + LINC00039) (a), antibody-only controls (protein name + none) (b), other negative controls including no-probe-no-antibody control (none + none), RNA probe-only control (none + LINC00339), and four RNA-protein pairs not included in HuRPA (GFP + LINC0039, CD40 + LINC0039, CD32 + LINC0039, LTBR + LINC0039 (c). Quantification is provided in Fig. 3k. Blue: DAPI staining. Red: RNA-PLA signal. Scale bar = 20 μm. (d) ROC curve plotting the true positive rate (TPR, y-axis) against the false positive rate (FPR, x-axis), using HuRPA as the reference dataset and RNA-PLA as the test dataset, while varying the threshold applied to RNA-PLA signals.
