Extended Data Fig. 3: Large-area montages of pan-ExM-t data from cortical and hippocampal regions. | Nature Biotechnology

Extended Data Fig. 3: Large-area montages of pan-ExM-t data from cortical and hippocampal regions.

From: All-optical visualization of specific molecules in the ultrastructural context of brain tissue

Extended Data Fig. 3: Large-area montages of pan-ExM-t data from cortical and hippocampal regions.The alternative text for this image may have been generated using AI.

a, Schematic of sample preparation. A coronal hemi-brain section (100 µm thick) was expanded and stained using the pan-ExM-t protocol. b, Wide-field fluorescence image of Thy-1 GFP expression. c, Wide-field phase contrast image after ~10x expansion. d, Wide-field, phase contrast image after ~20x expansion. Sample ROI corresponds to the red box shown in b,c. Letters indicate approximate regions corresponding to high-resolution confocal imaging shown in e-l. e, f, g, pan-ExM montage of cortical neuropil. Progressive zooms shown in f,g, show details. h, pan-ExM image of cortical white matter region. i, pan-ExM image of pyramidal cell layer of the hippocampus. j,k,l, pan-ExM montage of hippocampal neuropil. Progressive zooms shown in k,l, show details. All scale bars: not corrected for the expansion factor.

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