Extended Data Fig. 6: MST validation and binding affinity of the MYC IRES and corresponding compounds.
From: Predicting small molecule–RNA interactions without RNA tertiary structures
a. MST validation for the binding of 15 compounds with the MYC IRES across different prediction intervals. The colors of the outer frame represent various prediction intervals, with the response amplitude labeled in the frame. The CAS number, predicted binding score, and S/N ratio are listed below. Results are presented using normalized fluorescence, except for those marked by black dashed boxes, which highlight interactions that altered initial fluorescence but passed the SDS denaturation test (SD-test), with results shown using initial fluorescence. b. Binding affinity of 15 compounds across different prediction intervals and the MYC IRES. The colors of the outer frame represent various prediction intervals.
