Extended Data Fig. 6: Additional examples of evolutionarily conserved SVP events in human DLPFC, related to Fig. 5.
From: Mapping isoforms and regulatory mechanisms from spatial transcriptomics data with SPLISOSM
(a) Relationship between total read coverage in TREND regions per spot (x-axis) and proportion of significant spatially variably processed (SVP, top) or variably expressed (SVE, bottom) genes in all tested genes. Line and shading indicate fitted linear model and 95% confidence interval. (b) Number and proportion of human DLPFC SV genes that have spatially variable mouse homologs, grouped by recurrence (number of significant DLPFC samples, x-axis). (c) Sequencing depth of SVP and SVE genes, grouped by conservation and recurrence. Per-group sample size is indicated in (b). Boxplots show median (center line), interquartile range (box), and 1.5× interquartile range (whiskers). Group means are compared using two-sided T-test. (d) Pathway enrichment analysis comparing human SVP versus human SVENP genes. Related to Fig. 5f. (e) Spatial log-normalized expression of selected RBPs in sample 151673. (f) RBP-SVP associations ranked by recurrence (x-axis) and minimal GLMM p-values across samples (y-axis). Top potential regulators of MAP4 are highlighted and colored by whether the association is conserved in mouse. (g) MAP4 transcript structure and spatial log-normalized expression in human sample 151673.
