Extended Data Fig. 1: Spatially variable TREND events and their regulation in adult mouse brain, related to Fig. 4.
From: Mapping isoforms and regulatory mechanisms from spatial transcriptomics data with SPLISOSM
(a) HSIC-IR test agreements between Visium CBS (y-axis) and Slide-seqV2 hippocampus (x-axis) datasets. Points colored by expression perplexity. (b) Differential protein-altering alternative polyadenylation events between neurons and glial cells. Related to Fig. 4e. (c) Additional protein-altering TREND events and their spatial log-normalized expression. (d) Top RBPs ranked by number of significantly associated targets, color-coded by available motifs (CISBP-RNA) or CLIP data (POSTAR3), core APA machinery involvement and motif enrichment near SVP TREND events. Spatial log-normalized expression of selected RBPs shown at right. (e) PCBP2 transcript structure in mouse and human, along with eCLIP data from ENCODE. (f) PCBP2 isoform expression in spatial mouse CBS data (top) and across GTEx human tissues (bottom). In each scatter plot, Pearson’s R correlation coefficient and the associated p-value are displayed along with the fitted linear model and its 95% confidence interval.
