Extended Data Fig. 4: dpCoA-TLC analysis of RNA from Arabidopsis. | Nature Biotechnology

Extended Data Fig. 4: dpCoA-TLC analysis of RNA from Arabidopsis.

From: Quantification and transcriptome profiling reveal abundant, dynamic and translatable dephospho-CoA-capped RNAs

Extended Data Fig. 4: dpCoA-TLC analysis of RNA from Arabidopsis.

a-c, Detection of dpCoA-RNA in total RNA, isolated from inflorescence (a), shoot (b), root (b) and rosette leaf (c) using dpCoA-TLC. d-h, Detection of dpCoA-RNA in poly(A) RNA, short and long non-poly(A) RNA, isolated from inflorescence (d), rosette leaf (e), shoot (f) and root (g) using dpCoA-TLC. The [α-32P]-dpCoA cap signal was visualized with a Typhoon phosphorimager. Quantification of the detected signals is presented as a bar plot in Fig. 3e.

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