Extended Data Fig. 5: dpCoA-TLC analysis of RNA from mouse. | Nature Biotechnology

Extended Data Fig. 5: dpCoA-TLC analysis of RNA from mouse.

From: Quantification and transcriptome profiling reveal abundant, dynamic and translatable dephospho-CoA-capped RNAs

Extended Data Fig. 5: dpCoA-TLC analysis of RNA from mouse.

a, Detection of dpCoA-RNA in total RNA, isolated from mouse heart, brain and liver, using dpCoA-TLC. b-h, Detection of dpCoA-RNA in poly(A) RNA (b-d), short and long non-poly(A) RNA (e-h), isolated from mouse kidney, heart, brain and liver, using dpCoA-TLC. The [α-32P]-dpCoA cap signal was visualized with a Typhoon phosphorimager. Quantification of the detected signals is presented as a bar plot in Fig. 3f.

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