Extended Data Fig. 4: Normalized editing activity for ABEs across 27 endogenous sites, broken down by target A position.
From: Precise, minimally evolved adenine base editors generated through mutation reversion analysis
HEK293T cells were transfected with an ABE plasmid encoding ABE7.10 or variant editor indicated along with one of the 27 gRNA plasmids from Supplementary Fig. 18, or a non-target (NT) gRNA. Cells were harvested after 72 h and genomic loci of interest analyzed for editing activity with NGS. Within a given transfection replicate, absolute editing efficiency for all A’s was normalized to the editing activity of ABE7.10 at the H2.0 A5 position (the maximally edited A, across all sites and in every replicate). The normalized editing efficiencies for all 205 A’s are shown as a function of A position within the protospacer. Data are represented by box and whiskers plot where center shows median, hinges show quartiles, and whiskers show minimum and maximum. ABE7.10-derived and ABE8-derived reversion editors were tested in n = 3 biological replicates, ABE7.10, ABE8e, and ABE8.20 in n = 6. All H2.0 normalized editing values from all replicates are indicated with overlaying points. The horizontal dotted line indicates the 15% relative editing efficiency cut-off used to demarcate the editing window for each editor.
