Extended Data Fig. 7: Editing activity at 6 endogenous genomic sites in HeLa cells.
From: Precise, minimally evolved adenine base editors generated through mutation reversion analysis
HeLa cells were transfected with plasmids encoding an ABE (ABE-P2A-mCherry architecture) and a gRNA with the indicated protospacer sequence targeting an endogenous site, or a non-targeting (NT) control. Cells were allowed to incubate for 72 h. To normalize for variations in transfection efficiencies and low overall transfection efficiencies, cells were then sorted for mCherry fluorescence marker prior to harvest of genomic DNA and amplification and analysis of loci of interest. Only A’s where editing for one of the editors tested was greater than 1% are shown. Bars show the average of n = 3 independent transfection biological replicates, error bars show standard deviation, points show individual replicates.
