Extended Data Fig. 8: Resolution analysis for me4Pi-SMLM in whole-cell mitochondrial imaging.
From: Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging
a–c, Lateral localization precision (a), axial localization precision (b) and modulation depth (c) for the mitochondria image shown in Fig. 4f. d, DAFL 3D precision for molecules from each optical section. e, FRC resolution for each optical section. f, 3D localization distributions for molecules emitting ≥10 frames. The photon count for these molecules is 6,422 ± 2,362 (mean ± s.d.). g, Histograms of the distributions in f were fit with Gaussian functions, with fitted standard deviations reported.
