Extended Data Fig. 9: me4Pi-SMLM imaging of microtubules with fast imaging mode.
From: Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging
a, me4Pi-SMLM image of microtubules in a fixed COS-7 cell recorded with a cycle time of 9 ms. Color bar denotes depth. b, Cross-sections at locations denoted by the numbers in a, reconstructed with me4Pi-SMLM and 3D-SMLM. c, DAFL localization precision in 3D for the data in a. d, FRC analysis in each dimension of the data in a. Scale bars: 2 μm (a), 50 nm (b). Representative images are shown from six independent experiments.
