Fig. 3: Two-colour me4Pi-SMLM imaging.

a, Two-colour me4Pi-SMLM image of ER and microtubules in a fixed COS-7 cell. The lower-left corner shows the depth colour-coded image, while the upper-right corner shows the merged image of the two labels. b, Zoomed-in image of the yellow boxed region in a. c, A 10 nm-thick x–y slice of the blue boxed region in a. d, A 500 nm-thick y–z cross-section at the dashed region and a 100 nm-thick x–z cross-section along the dashed line in b. e, Merged two-colour me4Pi-SMLM image of ER membrane and lumen in a fixed COS-7 cell. f, Zoomed-in image of the yellow boxed region denoted in e. The lower-left and upper-right corners show the KDEL labelling and Sec61β labelling, respectively. g. A 50 nm-thick x–y slice of the yellow boxed region denoted in e. h, The 200 nm-thick x–z cross-sections at locations denoted by the numbers in g. i, Intensity profile along the white line in g. Colour bar denotes depth. a.u., arbitrary units. Representative images are shown from two to three independent experiments.