Fig. 4: Whole-cell me4Pi-SMLM imaging.

a, 3D rendering of computationally isolated synaptonemal complexes in a fixed mouse spermatocyte imaged with me4Pi-SMLM. b, y–z cross-sections at the yellow dashed region in a. c, y–z view of a. d, Zoomed-in images of 19 synaptonemal complexes, computationally isolated from the entire mouse spermatocyte nuclei. e, Representative 3D views of two synaptonemal complexes shown in d. f, 3D whole-cell imaging of mitochondria in HeLa cells imaged with me4Pi-SMLM. g, x–y projection of f and the cross-sections at locations denoted by the numbers. The colour bar denotes depth. All cross-section slices are 100 nm thick. Representative images are shown from two independent experiments.