Fig. 6: me4Pi-SMLM imaging in brain slices.

a, me4Pi-SMLM image of mitochondria in a 30 μm-thick mouse brain slice at a depth of ~20 μm. b,c, 3D rendering of the boxed regions denoted in a. d,e, 3D rendering of the boxed regions from tissue imaging at depths of 0 μm and 10 μm, shown in Supplementary Fig. 7. Cross-sections at the dashed lines denoted by numbers in b, c, d and e. f, DAFL 3D precision of the mitochondria image at each depth. g, FRC resolution (averaged from xy, xz and yz) of the mitochondria image at each depth. h, me4Pi-SMLM image of ChR2 in a 30 μm-thick mouse brain slice at a depth of ~20 μm (Supplementary Video 8). i, 3D rendering of the boxed regions denoted in h. j,k, 3D rendering of the boxed regions from tissue imaging at depths of 0 μm and 10 μm, shown in Supplementary Fig. 8. l, Cross-sections at the dashed line denoted by numbers in i, j and k. m, DAFL 3D precision of the ChR2 image at each depth. n, FRC resolution (averaged from xy, xz and yz) of the ChR2 image at each depth. Colour bar denotes depth. Representative images are shown from two to three independent experiments.