Extended Data Fig. 1: Optical layout of me4Pi-SMLM.
From: Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging
See the Methods section for a detailed description. Laser 1: 405 nm (OBIS LX 100 mW, Coherent); Laser 2: 488 nm (OBIS LS 150 mW, Coherent); Laser 3: 552 nm (2RU-VFL-P-2000-552, MPB Communications); Laser 4: 642 nm (2RU-VFL-P-2000-642, MPB Communications). AOTF: acousto-optic tunable filter; SMF: single-mode fiber; DM1–DM5: dichroic mirrors; F1–F4: emission filters; L1–L7: lenses; M1–M14: mirrors; CL1 and CL2: cylindrical lenses; CAM1 and CAM2: sCMOS cameras.
