Extended Data Fig. 10: Phylogenetic analysis of annotated CPK proteins and the divergence between ZmCPK39 and ZmCPK38 in their interaction with ZmDi19.

a, The annotated 41 CPK proteins in maize, two IV subfamily CPKs in rice and 34 CPK proteins in Arabidopsis were used for phylogenetic analysis. b, Interaction between ZmCPK38 and ZmDi19 in yeast two-hybrid assay. The Y2H assay indicated that ZmCPK38 does not interact with ZmDi19. DDO, double dropouts, medium lacking Leu and Trp; QDO, quadruple dropouts, medium lacking Ade, His, Leu and Trp.