Extended Data Fig. 6: In situ HCR analysis of selected cortical layer marker genes.
From: A temporal cortex cell atlas highlights gene expression dynamics during human brain maturation
Expression of a, layer 1 markers AQP4, FABP7 and RELN and b, layer 4–6 markers RORB, CLSTN2 and TSHZ2 in frozen temporal cortex tissue sections from the same 31-year-old and 15-year-old donor tissue used for Visium. High magnification views of layer 1 in a indicate AQP4/RELN-positive cells (yellow arrowheads) and FABP7 positive cells (green arrowhead). In high magnification views of layer 4 in b in the 31-year-old tissue section, RORB/CLSTN2-positive (white arrowhead) and RORB/TSHZ2-positive cells (green arrowhead) are indicated. In high magnification views of layer 4 in b in the 15-year-old tissue section RORB/CLSTN2/TSHZ2-positive cells (white arrowheads) are indicated. Dashed white lines indicate layer boundaries. Solid white line indicates tissue edge. Scale bars are 100 µm in low magnification views (tile scan at 40x) and 20 µm in high magnification views (63x). Data is representative of 2 technical replicate sections analysed per sample.
