Extended Data Fig. 5: Supporting analyses for mosaic loss of Y chromosome.
From: Transcript-specific enrichment enables profiling of rare cell states via single-cell RNA sequencing
(a) FlowFISH cytometry gating scheme, including control (no probe, left) and four male (XY) donors of different ages. The percent of cells corresponding to MSY+ (red) and MSY- (blue) in each donor gate are shown as numeric values. (b) Empirical cumulative distribution plot of the percent of Y chromosome UMIs stratified by the captured PERFF-seq library. Percentages of cells with 0 MSY UMIs from the scRNA-seq library are noted. P-values are from a two-sided Kolmogorov–Smirnov test comparing the distributions of the positive and negative samples.
