Extended Data Fig. 6: Supporting analyses of nuclei enrichment from fresh and fixed tissues.
From: Transcript-specific enrichment enables profiling of rare cell states via single-cell RNA sequencing
(a) UMAP of adult mouse cerebellum atlas26, including cell types (top) and Mobp expression (bottom). The Mobp+ oligodendrocytes and oligodendrocyte precursors are circled with their frequency noted. (b) Reduced dimensionality representation of public GBM FFPE Flex data showing 17 clusters. (c) Annotation of marker genes for cluster 10, the population highlighted by the arrow in (b). (d) Supporting marker genes annotating other subpopulations from the PERFF-seq experiment, including the primary cluster of granule cells. (e) Additional marker genes from Mobp+ cells were profiled with PERFF-seq. Atp-associated genes (Atp1b1, Aqp4) supporting rare subclsuters are highlighted in the boxes as well as marker genes highly expressed in all cells. (f) Empirical cumulative distribution plot of the raw UMI counts for each of the three genes enriched via FlowFISH, stratified by the captured PERFF-seq library. (g) Additional marker genes showing heterogeneity defining subclusters of endothelial cells and pericytes.
