Extended Data Fig. 8: The PAN domain of SBRR1 and the DP-repeat domain of SIP1 are required for their interaction in the Y2H assay.
From: Natural variation in SBRR1 shows high potential for sheath blight resistance breeding in rice
a, Western blot detection for the expression of Myc (c-Myc epitope tag)/HA (hemagglutinin epitope tag) fusion proteins in yeast. These proteins (labeled on each of the figures) were used in Fig. 6a. By using Myc/HA antibodies, each of the Myc/HA fusion proteins was detected essentially as a single major band representing the full-length GFP fusion protein in rice protoplasts, consistent with the predicted protein size. b, Y2H assays for the interaction of truncated SBRR1 and SIP1 proteins. c, Western blot analysis for confirming the expression of Myc/HA fusion proteins in yeast. These proteins (labeled on each of the figures) were used in Extended Data Fig. 8b. By using Myc/HA antibodies, each of the Myc/HA fusion proteins was detected essentially as a single major band representing the full-length GFP fusion protein in rice protoplasts, consistent with the predicted protein size. d, In vitro GST pull-down assay for the interaction between the PAN domain of SBRR1 and DP-repeat domain (DP, aspartate-proline) of SIP1. Lower panel shows the protein abundance stained by CBB (coomassie brilliant blue) in SDS-PAGE gel. The Y2H and pull-down assays were done independently three times.
