Fig. 3: ShB resistance function, geographic distribution and molecular evolution of elite SBRR1-R.
From: Natural variation in SBRR1 shows high potential for sheath blight resistance breeding in rice
a, Expression levels of SBRR1 in EPro lines (pSBRR1XWX7:SBRR1DJ, EPro1/2/3, T3 generation) (n = 3). b, ShB resistance of EPro lines. Data in a and b are presented as means ± s. d. Statistical significance in a was determined by a two-sided Student’s t-test. Different lowercase letters in b indicate a significant difference (P < 0.05) based on one-way ANOVA with Duncan’s multiple range test. Scale bar, 5 cm. c, Geographic distributions of SBBR1-R and SBRR1-S among 2,478 cultivated rice varieties. Orange and green circles indicate SBRR1-R and SBRR1-S, respectively. d, The relative ratio of nucleotide diversity of cultivated rice to common wild rice in the whole rice chromosome 11. The dotted red line indicates the SBRR1 locus. The ratios of nucleotide diversity of japonica/O. rufipogon and indica/O. rufipogon are shown. The ratio in SBRR1 is higher than in flanking regions for japonica/O. rufipogon, whereas the ratio in SBRR1 is notably lower than in flanking regions for indica/O. rufipogon. e, The Tajima’s D analysis in the whole chromosome 11 of cultivated rice. Purple line and dots indicate the SBRR1 locus. The estimated Tajima’s D values for the SBRR1 locus are substantially positive in indica but near zero in japonica. f, Haplotype network of SBRR1 generated using the coding and upstream 1.5-kb sequences of SBRR1 from cultivated rice varieties and common wild rice. Black spots on the lines indicate mutational steps between two haplotypes.
