Fig. 7: SIP1 is required for transport of SBRR1 to the plasma membrane.
From: Natural variation in SBRR1 shows high potential for sheath blight resistance breeding in rice
a, Subcellular localization of SBRR1 and SIP1 in WT protoplasts. b,c, Subcellular localization of SBRR1-GFP in WT and sip1-ko1 protoplasts, and its partition in PM (plasma membrane) and ER (endoplasmic reticulum). d,e, Subcellular localization of SBRR1ΔPAN-GFP (PAN, plasminogen-apple-nematode) in WT protoplasts and its partition in PM and ER. The images in a, b and d were taken under a laser scanning confocal microscope 12 h after transfection. Scale bar (a, b and d), 10 μm. The ratio of protoplasts with only PM-localized GFP signals to the total protoplasts with GFP signals was measured with 100 fluorescent cells in c and e. The protoplasts without clear PM-localized GFP signals show ER-localized GFP signals. Data in c and e are presented as means ± s.d (n = 4). Different lowercase letters in c indicate significant differences (P < 0.05) based on one-way ANOVA with Duncan’s multiple range test. Statistical significance in e was determined by a two-sided Student’s t-test. f, SBRR1 protein distribution in two-phase partitioning. S, soluble fraction; M, microsomal fraction; U, upper phase of partitioning; L, lower phase of partitioning. The blot was probed with the antibody indicated to the right of each panel. PIP1;1 (plasma membrane intrinsic protein 1;1), plasma membrane marker; HSP82 (heat shock protein 82), cytoplasmic marker. The subcellular localization and two-phase partitioning assays were done independently three times.
