Extended Data Fig. 8: Molecular characterization of Pb effector PbPDIa. | Nature Genetics

Extended Data Fig. 8: Molecular characterization of Pb effector PbPDIa.

From: Inactivation of β-1,3-glucan synthase-like 5 confers broad-spectrum resistance to Plasmodiophora brassicae pathotypes in cruciferous plants

Extended Data Fig. 8: Molecular characterization of Pb effector PbPDIa.The alternative text for this image may have been generated using AI.

a, The protein diagram of PbPDIa, SP: signal peptide. b, The relative expressions of PbPDIa during Pb infection. The expression level of PbPDIa at 8 dpi was used as the control. c, Validation of PbPDIa secretion in yeast. CMD-W: sucrose and glucose media lacking tryptophan. YPRAA: raffinose medium. This experiment was repeated twice with a similar result. d, Validation of PbPDIa secretion by TTC staining. TTC: 2,3,5-triphenyltetrazolium chloride, reduced by glucose to red 1,3,5-tribenzoic acid. This experiment was repeated twice with a similar result. In b, data were presented as mean ± s.d. and the statistical significance were determined by a two-sided Student’s t test.

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