Extended Data Fig. 4: Bias against asRT identification varies across cell lines.
From: Genetic variants affecting RNA stability influence complex traits and disease risk
a, Number of expressed genes with and without heterozygous genic single-nucleotide variants (SNVs). Genes without heterozygous SNVs are further categorized into whether they have intronic heterozygous SNVs or 0 heterozygous SNVs (even when introns are considered). To be considered expressed, a gene must have average base coverage ≥ 10 across all genic regions, across all 6 timepoint samples. b, Prevalence (top panel) and number (bottom panel) of intron-based asRT genes. Prevalence is calculated using the total number of genes that are testable based on having SNVs in intronic regions. c, Comparison of the prevalence of stability-regulated genes versus transcriptionally regulated genes (including and excluding intron-based asRT genes). Stability regulated genes include asRS and mixed genes. Transcriptionally regulated genes include asRT, intronic asRT, and mixed genes. To calculate prevalence, the number of genes falling under each of these categories is summed and divided by the total number of genes that were tested using RNAtracker in the cell line.
