Extended Data Fig. 5: Heterogeneity of macrophages across tissues. | Nature Genetics

Extended Data Fig. 5: Heterogeneity of macrophages across tissues.

From: A multi-tissue single-cell expression atlas in cattle

Extended Data Fig. 5: Heterogeneity of macrophages across tissues.

(a) Heatmap shows the average regulatory activity z-score of 245 TFs of macrophages in 29 tissues. (b) Heatmap represents the key metabolic pathways enriched by macrophage subtypes. (c) Box plot shows the gene signature scores of MHC I (21 genes) and MHC II (26 genes) in macrophage subtypes (sample size = 11), calculated by AddModuleScore in Seurat 4.0.6. The central band in the boxplot represents the median, the box boundaries represent the 25% to 75% percentiles, and the whiskers extend 1.5 × the interquartile range. Statistical test was calculated based on Wilcoxon test with two-sided. The same sample size and statistical test were applied to the following Figures d-f. (d) Box plot shows the scores of M1 (14 genes) and M2 (16 genes) in macrophage subtypes, calculated by AddModuleScore of Seurat. (e) Box plot shows the scores of M1 and M2 in macrophage in 40 tissues, calculated by AddModuleScore of Seurat. (f) Box plot shows the pseudotime of each macrophage subtype.

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