Fig. 8: Cross-species comparisons and disease associations.
a, MetaNeighbor area under the receiver operating characteristic (AUROC) values calculated using shared genes of the same cell types in cattle and humans. b,c, Regulon specificity score (RSS) of TFs in immune (b) and epithelial (c) cells. The scatter plot represents TFs that were identified in both cattle and humans; the x axis shows the RSS of each TF in cattle and the y axis shows the RSS in humans. Representative TFs were considered significant (RSS > 0.25) and are color-labeled. The r represents the Pearson correlation coefficient (PCC) measuring the linear association of RSS values between the two species. The corresponding P value was calculated based on a two-sided t-test and adjusted for multiple comparisons (FDR). d–g, Cellular communication in the small intestine (d,e) and liver (f,g) between cattle and humans. The network diagram shows the analysis of cellular communication between cattle and humans; the column stacking diagram represents the proportion of the two species in the signaling pathway. Red represents pathways significantly upregulated in cattle, while blue represents pathways significantly upregulated in humans; black represents pathways that are not significant in either cattle or humans. h,i, Heritability enrichment of cell types for orthologous marker genes with MS (h) and IBD (i). The y axis represents the significance level (−log10(P)) of heritability enrichment and the x axis represents all cell types identified in the corresponding cattle tissue. P values were derived from the heritability enrichment analysis and adjusted for multiple comparisons (FDR). The red dashed line represents the significance threshold of FDR = 0.05. The figure was created with BioRender.com.
