Extended Data Fig. 3: Gene regulatory impact of cBAF restoration in DDEC cell lines and primary tumors. | Nature Genetics

Extended Data Fig. 3: Gene regulatory impact of cBAF restoration in DDEC cell lines and primary tumors.

From: Shifted assembly and function of mSWI/SNF family subcomplexes underlie targetable dependencies in dedifferentiated endometrial carcinomas

Extended Data Fig. 3: Gene regulatory impact of cBAF restoration in DDEC cell lines and primary tumors.

A. Immunoblot for indicated mSWI/SNF components following ARID1A restoration (72 h). B. PCA plots performed on RNA-seq in AN3CA and VOA1066 cell lines following ARID1A restoration (n = 3 experimental replicates). C. Heatmaps indicating clustering of -Dox and +Dox (+HA-ARID1) conditions. D. GSEA analyses performed on AN3CA and VOA cells (ARID1A rescue/no rescue); NES, Normalized Enrichment Score. E. Clustered heatmap indicating genes up and downregulated following ARID1A rescue. F. Venn diagrams indicating cell line-specific and shared up- and down-regulated genes upon ARID1A rescue. G. Volcano plots showing up- (red) and down- (blue) regulated proteins (by TMT-MS) following 72 h of ARID1A restoration in AN3CA and VOA1066 DDEC cell lines. H. Representative ChIP-seq and ATAC-seq tracks at the CDH1, CLDN4, TONSL, and BRCA2 gene loci with corresponding bar graphs depicting gene expression (RNA-seq).

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