Extended Data Fig. 4: The downstream genes regulated by GhTT2_A07 and GhPAR. | Nature Genetics

Extended Data Fig. 4: The downstream genes regulated by GhTT2_A07 and GhPAR.

From: Integrated metabolomic and transcriptomic analyses identify MYB genes regulating key metabolites and agronomic traits in upland cotton Gossypium hirsutum

Extended Data Fig. 4: The downstream genes regulated by GhTT2_A07 and GhPAR.

a, The Yeast One-Hybrid (Y1H) assay indicates that GhTT2_A07 binds to the promoter regions of GhG6PDH, GhSK1, GhADT6, GhDFR, GhANR, GhLAR, and GhGT. b, The electrophoretic mobility shift assay (EMSA) has confirmed GhTT2_A07’s binding to the promoters of GhSK1, GhANR, and GhLAR in vitro. c, The Yeast One-Hybrid (Y1H) assay demonstrates GhPAR’s binding to the promoter regions of GhDFR, GhANR, GhLAR, and GhGT. d, The electrophoretic mobility shift assay (EMSA) confirms GhPAR’s binding to the promoters of GhDFR, GhANR, GhLAR, and GhGT in vitro. Each experiment was conducted with three independent biological replicates (a-d).

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