Extended Data Fig. 6: Generation and characterization of GhPAR knockout (GhPAR-KO) lines using CRISPR/Cas9. | Nature Genetics

Extended Data Fig. 6: Generation and characterization of GhPAR knockout (GhPAR-KO) lines using CRISPR/Cas9.

From: Integrated metabolomic and transcriptomic analyses identify MYB genes regulating key metabolites and agronomic traits in upland cotton Gossypium hirsutum

Extended Data Fig. 6: Generation and characterization of GhPAR knockout (GhPAR-KO) lines using CRISPR/Cas9.

a, CRISPR/Cas9-mediated generation of GhPAR-KO lines using two distinct single-guide RNAs (sgRNA1 and sgRNA2), with the protospacer-adjacent motif (PAM) highlighted in blue. b. Comparison of fiber strength and fiber micronaire between the wild types, GhPAR-KO-1 lines and GhPAR-KO-4 lines. c, Sequence alterations in GhPAR-KO lines, with deletions and insertions represented by dashes and red texts, respectively. d. qRT-PCR analysis comparing the expression levels of GhDFR, GhANR, GhLAR, and GhGT in ovules (5 DPA) of the wild type, GhPAR-KO-1 lines and GhPAR-KO-4 lines. e, Quantification of various auxins and gibberellin (GA) in ovules (5 DPA) of the wild type, GhPAR-KO-1 lines and GhPAR-KO-4 lines. Data are presented as mean values +/- SD (b, d). Statistical significances are determined using a two-tailed Student’s t-test (b, d). All comparative analyses were conducted with three independent biological replicates (b, d-e).

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