Extended Data Fig. 9: RCN2 interacts with rice SPL family proteins through the SBP domain.
From: Precise control of chromatin loop extrusion enhances sustainable green revolution yield in rice
a, BiFC assays. Constructs expressing N-terminus of YFP-tagged OsSPL family proteins and C-terminus of YFP-tagged RCN2 protein were co-transformed into rice protoplast. Scale bar, 10 µm. b, Schematic diagrams of OsSPL14 protein, SBP domain and mutant version of OsSPL14ΔSBP that lacks the SBP domain. c, BiFC assays. Construct expressing N-terminus of YFP-tagged SBP domain or deletion mutant version of OsSPL14ΔSBP, together with the construct expressing C-terminus of YFP-tagged RCN2, were co-transformed into rice protoplast. Scale bar, 10 µm. Panels in a and c (from left to right), YFP signals, differential interference contrast (DIC) images, merged channels. All these experiments were repeated independently three times with similar results.
