Extended Data Fig. 6: Effects of TGFBR1 inhibition and αPD-L1 on TAMs. | Nature Genetics

Extended Data Fig. 6: Effects of TGFBR1 inhibition and αPD-L1 on TAMs.

From: TGF-β builds a dual immune barrier in colorectal cancer by impairing T cell recruitment and instructing immunosuppressive SPP1+ macrophages

Extended Data Fig. 6: Effects of TGFBR1 inhibition and αPD-L1 on TAMs.

a, Canonical marker gene expression across all tumor-associated macrophage (TAM) subsets. Dot size indicates the proportion of cells expressing each gene; color reflects expression centered to the average across all TAMs (scale: −1 to 2). b, Canonical marker gene expression in C1q+ and Spp1+ TAM subsets, centered to their subset averages (scale: −2 to 2). Dot size represents the proportion of expressing cells per cluster. c, Proportions of TAM subsets across treatments (vehicle, αPD-L1, galunisertib, dual). Mice per group: vehicle, n = 4; αPD-L1, n = 2; galunisertib, n = 2; dual, n = 2. Horizontal lines indicate medians. d, UMAP of TAMs showing Trem2 expression across all treatments. Color scale reflects expression level. e, Volcano plot showing differentially expressed genes in TAMs between vehicle- and αPD-L1–treated groups (10x Genomics). Genes with P < 0.05 and log2(fold change) ≥ 0.85 are considered significant. NCM, non-classical monocytes; TAM, tumor-associated macrophages.

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