Extended Data Fig. 3: Cell-type-specific regulons in the scRNA-seq datasets.
From: Spatially resolved multi-omics of human metabolic dysfunction-associated steatotic liver disease
a, Regulon specificity score for each annotated cell population. The top five regulons in each cell type are highlighted in red and labeled on the plot. The specificity score is shown on the Y axis. b, AUC distribution across cells for the MITF regulon. The calculated binary threshold is shown as a red dotted line. c, Expression of MITF gene in scRNA-seq dataset displayed in UMAP space. d, Stacked violin plot displaying the top 50 putative MITF-targeted gene expression levels in each cell subset, ordered by descending regulation weight. Red arrows indicate LAM-specific genes. e, Quantification of marker genes of LAMs by RT-qPCR in THP-1 cells after MITF knockdown (si-MITF) by small interfering RNA for 24 h (n = 6, biological replicates). f, AUC distribution across cells for PPARG regulon. The calculated binary threshold is shown as a red dotted line. g, Quantification of PGC1α, PPARγ, CPT1A, ACADVL and CYP21A1 by RT-qPCR in THP-1 cells after si-MITF for 24 h (n = 6, biological replicates). Data are presented as mean values ± s.d., statistical significance was determined by unpaired two-sided Student’s t-test.
