Extended Data Fig. 6: Identification of spatial metabolic modules by Hotspot and alignment with ST via STalign.
From: Spatially resolved multi-omics of human metabolic dysfunction-associated steatotic liver disease
a, Rearrangement of the spatial coordinates n = 27 MALDI-MSI SM samples in the two-dimensional coordinate system. b, Spatial distribution of Hotspot-identified Module 13 across twelve representative tissue sections. c, 47 metabolites from Module 13 are presented as heatmap, with columns representing pixels within each disease status and rows displaying metabolite annotations. d, Overview of the alignment steps between MSI data points and H&E image of Visium ST by STalign. e, Key parameters that indicate the effects of spatial multimodal alignment.
