Fig. 4: Effects of RP variants in RNU4-2 and RNU6 on snRNP maturation.
From: De novo and inherited dominant variants in U4 and U6 snRNA genes cause retinitis pigmentosa
a,b, Immunoprecipitation of U4-MS2 (WT and variants) (a) and U6-MS2 (WT and variants) (b). snRNPs were immunoprecipitated via MS2-YFP by anti-GFP antibodies and co-precipitated proteins were detected by western blotting. The position of the MS2 loop (green) in snRNAs is indicated. Four independent experiments were quantified. Immunoprecipitated proteins are normalized to input and U4 or U6 WT controls. Middle bars indicate average values and error bars the s.e.m. Statistical significance was analyzed by the two-tailed unpaired t-test and the P values were adjusted using the Benjamini–Hochberg FDR method to control for false discoveries. P values ≤ 0.05 are indicated. Full-length blots and antibody validation are provided as Source Data. Ctrl, control; IP, immunoprecipitation; WT, wild type.
