Extended Data Fig. 1: Ang-Tie2 complex formation occurs in a sulfation-dependent manner.
From: Sulfated glycans engage the Ang–Tie pathway to regulate vascular development
Binding of biotinylated HS sulfation motifs to immobilized Tie2 in the presence or absence of a Ang1 or b Ang4, as detected by ELISA. Removal of the N- and O-sulfate groups (De-N,O), the N-sulfate groups (De-N), the O-sulfate groups (De-O), the 6-O-sulfate groups (De-6O), or the 2-O-sulfate groups (De-2O), or conversion of the N-sulfate to N-acetyl groups (N-Ac) reduced formation of HS-Ang1/4-Tie2 ternary complexes. Data represent mean ± s.e.m.; n = 2 independent replicates.
