Extended Data Fig. 3: HS engages Tie1 in a sulfation-specific manner.
From: Sulfated glycans engage the Ang–Tie pathway to regulate vascular development
Binding of Tie1-Fc to different HS sulfation motifs, as determined by ELISA. Removal of the N- and O-sulfate groups (De-N,O), the O-sulfate groups (De-O), or the 6-O-sulfate groups (De-6O), or conversion of the N-sulfate to N-acetyl groups (N-Ac) abolished the HS–Tie1 interaction. Removal of the N-sulfate groups (De-N) or the 2-O-sulfate groups (De-2O) greatly reduced HS-Tie1 binding. Data represent mean ± s.e.m.; n = 2 independent replicates.
